irf4  (Cell Signaling Technology Inc)

Journal: Cell Death & Disease

Article Title: Plasmodium yoelii infection induces lung injury by modulating type 2 conventional dendritic cells autophagy via the STAT3-IRF4 signaling

doi: 10.1038/s41419-026-08675-4

Figure Lengend Snippet: The lung cells of normal mice were cultured with PGN (50 μg/mL), Poly(I: C) (50 μg/mL), LPS (50 ng/mL), R848 (100 ng/mL), and the level of p-STAT3 ( A ) and IRF4 ( B )of pulmonary cDC2 were detected by FCM. The lung cells of normal mice were cultured with PGN, Poly(I: C), LPS, R848 and JSI-124; the percentage of cDC2 was detected by FCM ( C , D ). The lung cells of normal mice were cultured with iRBC lysate, CU-CPT 4a (10 μM) and Enpatoran (1 μM), and the percentage of cDC2 was detected by FCM ( E ). Date were presented as the mean ± SD and representative of three independent experiments, *, P < 0.05；**, P < 0.01；***, P < 0.001.

Article Snippet: IRF4 Rabbit pAb (A0524), LC3B Rabbit mAb (A19665), and p62 Rabbit mAb (A19700) were purchased from ABclonal (Beijing, China).

Techniques: Cell Culture

Journal: Cell Death & Disease

Article Title: Plasmodium yoelii infection induces lung injury by modulating type 2 conventional dendritic cells autophagy via the STAT3-IRF4 signaling

doi: 10.1038/s41419-026-08675-4

Figure Lengend Snippet: CD11c + DCs were isolated from normal and infected mouse lung cells, and the expression of IRF4 was detected by RT-qPCR ( A ). The proportion and average fluorescence intensity of IRF4 in pulmonary cDC2 from the normal and infected mice were detected by FCM ( B ). Representative images of lung tissues ( C ). The weights of the lungs ( D ) and organ coefficient ( E ) were compared between the two experimental groups. Representative images of H&E staining of lung tissues. Blue arrows indicate alveolar morphology, while black arrows point to leukocyte infiltration. Scale bars represent 200 μm ( F ). At 8 days post-infection, mice were euthanized, lung cells were collected, and the percentage and absolute number of cDC2 were determined by FCM, ( n = 6) ( G ). Single cell suspension was isolated from the lungs of normal mice and treated with iRBC lysate and SIM ( H ). Measure the proportion and absolute number of cDC2, activated molecule CD86 ( I ), and cytokine IL-12 ( J ) with or without the addition of IRF4 inhibitor SIM(1 μg/mL). Date were presented as the mean ± SD and representative of three independent experiments, *, P < 0.05；**, P < 0.01；***, P < 0.001.

Article Snippet: IRF4 Rabbit pAb (A0524), LC3B Rabbit mAb (A19665), and p62 Rabbit mAb (A19700) were purchased from ABclonal (Beijing, China).

Techniques: Isolation, Infection, Expressing, Quantitative RT-PCR, Fluorescence, Staining, Single Cell, Suspension

Journal: Cell Death & Disease

Article Title: Plasmodium yoelii infection induces lung injury by modulating type 2 conventional dendritic cells autophagy via the STAT3-IRF4 signaling

doi: 10.1038/s41419-026-08675-4

Figure Lengend Snippet: CD11c + DCs from the lungs were sorted for immunofluorescence detection ( A ) and western blot ( B ). Single-cell suspensions were isolated from the lungs of normal mice and treated with iRBC lysis. After treatment with JSI-124 (500 nM), SIM (1 μg/mL), BafA1 (100 nM) and Rapa(5 nM), the proportion ( C ) of cDC2, cytokine IL-12 production ( D ), and phagocytic ability ( E ) were measured. The lung cells of normal mice were cultured with iRBC lysates and JSI-124, the expression of IRF4 was detected by FCM ( F ). The lung cells of normal mice were cultured with iRBC lysates and SIM, the expression of p-STAT3 was detected by FCM ( G ). Date were presented as the mean ± SD and representative of three independent experiments, *, P < 0.05；**, P < 0.01；***, P < 0.001.

Article Snippet: IRF4 Rabbit pAb (A0524), LC3B Rabbit mAb (A19665), and p62 Rabbit mAb (A19700) were purchased from ABclonal (Beijing, China).

Techniques: Immunofluorescence, Western Blot, Single Cell, Isolation, Lysis, Cell Culture, Expressing

Journal: Cell Death & Disease

Article Title: Plasmodium yoelii infection induces lung injury by modulating type 2 conventional dendritic cells autophagy via the STAT3-IRF4 signaling

doi: 10.1038/s41419-026-08675-4

Figure Lengend Snippet: Plasmodium infection inhibited lung cDC2 autophagy through the TLR3/7-STAT3-IRF4 signaling pathway, resulting in pathological damage and compromised parasite clearance within mouse lungs. Elevated IL-12 secreted by cDC2 promoted Th1 differentiation, which induced detrimental pulmonary inflammation. Targeting STAT3 or IRF4 restored cDC2 autophagy, crippled their abnormal expansion, and mitigated Th1 cell-mediated inflammation, thereby attenuating lung damage.

Article Snippet: IRF4 Rabbit pAb (A0524), LC3B Rabbit mAb (A19665), and p62 Rabbit mAb (A19700) were purchased from ABclonal (Beijing, China).

Techniques: Infection

Journal: Bioactive Materials

Article Title: Immunomodulatory supramolecular hydrogel for rheumatoid arthritis management via adenosine A2A receptor-mediated macrophage remodeling

doi: 10.1016/j.bioactmat.2025.11.031

Figure Lengend Snippet: Macrophage remodeling via MTX@PNSH therapy elicits the reestablishment of immune homeostasis at the transcriptional level. (A) qRT-PCR analysis of relative mRNA of Irf3 with different treatments. (B) qRT-PCR analysis of relative mRNA of Irf4 with different treatments. (C) qRT-PCR analysis of relative mRNA of Irf5 with different treatments. (D) qRT-PCR analysis of relative mRNA of Irf8 with different treatments. (E) Heatmap analysis was performed to compare the gene expression of Irf3 , Irf4 , Irf5 , and Irf8 across different treatment groups. (F) Western blotting analysis of IRF4, and IRF8 levels in macrophages after 24 h of different treatment. (G) Quantitative analysis of IRF4 protein expression. (H) Quantitative analysis of IRF8 protein expression. Data are presented as mean ± SD, n = 3. Statistical significance was determined using one-way ANOVA, followed by Dunnett's post hoc test for comparisons between groups.

Article Snippet: Subsequently, 10 mg of protein was loaded into 10 % SDS-polyacrylamide gel electrophoresis for detection of adenosine A2AR (sc-32261, Santa Cruz), IRF4 (#62834S, Cell Signaling Technology, MA, USA), IRF8 (#5628T, Cell Signaling Technology, MA, USA) and GAPDH (TA-08, ZSGB-BIO, Beijing, China).

Techniques: Quantitative RT-PCR, Gene Expression, Western Blot, Expressing